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- Title
Minimal interleukin expression in canine intracranial meningiomas.
- Authors
Al-Nadaf, S.; Platt, S. R.; Kent, M.; Northrup, N.; Howerth, E. W.
- Abstract
Context The propagation of meningiomas has been linked with several inflammatory interleukins (ILs). Human meningioma cells have been found to synthesise and secrete IL-6, as well as to express IL-8 mRNA. The aim of this study was to determine whether canine intracranial meningiomas express IL-6 and/or IL-8. Main conclusion The limited IL-6 expression and lack of IL-8 expression indicated that IL-6 and IL-8 inhibitors were unlikely to be beneficial for the treatment of canine meningiomas. The limited IL expression may be because of species differences, fewer high-grade tumours or sample size. Approach Nineteen surgically excised formalin-fixed, paraffin-embedded canine intracranial meningioma biopsy samples were collected. Immunohistochemical detection of IL-6 and IL-8 was performed by an automated staining system using anti-canine IL-6 and anti-canine IL-8 monoclonal antibodies, respectively. To quantify IL-6 and IL-8, staining of the entire meningioma section was evaluated for percentage of cells positively stained and their staining intensity. The percentage of cells positively stained was recorded as: 0 per cent of cells stained; 1 to 30 per cent of cells stained; 31 to 60 per cent of cells stained; and 61 to 100 per cent of cells stained. Staining intensity was subjectively recorded as: 0, negative expression; 1, weak expression; 2, moderate expression; and 3, marked expression. Results Of the 19 tumour samples, staining for IL-6 expression was positive in five samples and IL-8 expression was positive in zero samples. IL-6 staining in the positive tumour samples was seen as diffusely cytoplasmic. In all five IL-6 positively stained samples, the percentage staining area was greater than 61 per cent; two of the five positive samples had weak staining intensity, while the remaining three positive samples had moderate staining intensity. Interpretation The limited IL-6 expression and lack of IL-8 expression indicates that IL-6 and IL-8 inhibitors are unlikely to be beneficial for the treatment of canine meningiomas. However, it should be noted that a failure to detect an antigen with immunohistochemistry may not be equivalent to a lack of expression. Significance of findings This study showed that the role of ILs in the pathophysiology of canine intracranial meningiomas seems to be limited.
- Subjects
MENINGIOMA in animals; DOG diseases; INTERLEUKINS; GENE expression; MESSENGER RNA; BIOPSY; IMMUNOHISTOCHEMISTRY; MONOCLONAL antibodies
- Publication
Veterinary Record: Journal of the British Veterinary Association, 2015, Vol 177, Issue 3, p75
- ISSN
0042-4900
- Publication type
Article
- DOI
10.1136/vr.103135