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- Title
Unique gene expression and clinical characteristics are associated with the 11q23 deletion in chronic lymphocytic leukaemia.
- Authors
Dickinson, John D.; Smith, Lynette M.; Sanger, Warren G.; Zhou, Guimei; Townley, Peter; Lynch, James C.; Steven Pavletic, Z.; Bierman, Philip J.; Joshi, Shantaram S.
- Abstract
Chromosome abnormalities influence prognosis and tumour progression in B-cell Chronic Lymphocytic Leukaemia (CLL). This study sought to determine whether these different disease subgroups were associated with unique gene expression patterns. Thirty-four cases of CLL were screened for the 11q23, 13q14, 17p13 deletions, and trisomy 12 by fluorescencein situhybridization (FISH). Expression of 205 cell signalling and apoptosis genes were compared by cDNA array among cases with different chromosome abnormalities. A majority of the statistically differentially expressed genes were present in the 11q23 deletion group by hierarchical clustering.CDC2, a serine/threonine kinase, was overexpressed in the 11q23 deletion group (P = 0·0004) and confirmed by TaqmanTM real-time polymerase chain reaction. Several other genes associated with cell signalling were overexpressed in the 11q23 deletion group. A strong overall correlation existed between the presence of different chromosome abnormalities and a number of prognostic factors including immunoglobulin heavy chain variable region mutation status (P = 0·011), time to treatment (P = 0·025) and lymphocyte doubling time (P = 0·034). This study confirmed the prognostic impact of chromosome abnormalities identified by FISH in CLL, particularly the 11q23 deletion and trisomy 12. In addition, the 11q23 deletion group was associated with a unique gene expression pattern involving cell signalling and apoptosis genes.
- Subjects
GENETIC mutation; CHROMOSOME abnormalities; KARYOTYPES; LYMPHOCYTIC leukemia; CANCER invasiveness; GENETIC regulation; CELL death
- Publication
British Journal of Haematology, 2005, Vol 128, Issue 4, p460
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/j.1365-2141.2004.05344.x