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- Title
Isolation and Purification of Proteins in Liver of Yak (Bos grunniens) and Its Antioxidant Activities.
- Authors
Liu Qing-haia; Guo Jie; Li Tian-cai
- Abstract
Seven proteins (BGP1, BGP2, BGP3, BGP4, BGP5, GBP6, and BGP7) were isolated respectively from the liver of yak (Bos grunniens) by homogenizing, step precipitating with ammonium sulfate, ion-exchange chromatography on DEAE-52 and gel filtration chromatography on Sephadex G-200. Each protein gave a single band on SDS-PAGE and the molecular weight of those proteins were from 97.2 KD to 116 KD. The anti-oxidant activities of proteins were evaluated using the method of chemistry luminescence, including 1,1-diphenyl-2-picrylhydrazyl (DPPH ·), superoxide (Due to image rights restrictions, multiple line equation(s) cannot be graphically displayed) and hydroxyl (middot; OH) radical scavenging assays. The proteins could scavenge superoxide radical, hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH ·). Especially, the effects of BGP5 and BGP1 on scavenging superoxide free radical were obvious, with scavenging ability 82.91% and 72.92% at the concentrations of 250 mg/L. The results indicated that proteins may be exploited as health food or functional food.
- Subjects
PROTEIN analysis; LIVER; YAK; ANTIOXIDANTS; AMMONIUM sulfate; ION exchange (Chemistry); MOLECULAR weights
- Publication
Natural Product Research & Development, 2012, Vol 24, Issue 5, p581
- ISSN
1001-6880
- Publication type
Article