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- Title
M-PEIs nanogels: potential nonviral vector for systemic plasmid delivery to tumor cells.
- Authors
Dong, L.; Xu, H.; Liu, Y.-B.; Lu, B.; Xu, D.-M.; Li, B.-H.; Gao, J.; Wu, M.; Yao, S.-D.; Zhao, J.; Guo, Y.-J.
- Abstract
Successfully systemic gene therapy has been hindered by vector-related limitations, including toxicity and inefficient gene delivery to tumor cells after intravenous administration. In this study, we evaluated the potential of spherical polyethylenimine nanogels (M-PEIs) as a novel vector for intravenous delivery of plasmids to tumor cells. M-PEIs provided a sustained release of plasmids up to 14 days and were also effective in protecting plasmids from enzymatic degradation in serum-conditioned media. M-PEIs showed no obvious cytotoxicity to mammalian cells in vitro as well as to liver, heart and kidney in mice after intravenous injection. Importantly, following intravenous administration of M-PEIs/plasmid complexes into human hepatocellular carcinoma xenograft-bearing mice, green fluorescence protein reporter gene expression was predominantly found in the tumor. This study indicates that M-PEIs may be a candidate for systemic delivery of plasmids into tumors.Cancer Gene Therapy (2009) 16, 561–566; doi:10.1038/cgt.2009.11; published online 30 January 2009
- Subjects
CANCER treatment; GENE therapy; LIVER cancer; CLINICAL trials; TOXICITY testing; INTRAVENOUS injections
- Publication
Cancer Gene Therapy, 2009, Vol 16, Issue 7, p561
- ISSN
0929-1903
- Publication type
Article
- DOI
10.1038/cgt.2009.11