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- Title
Micro‐ribonucleic acid expression signature of metastatic castration‐resistant prostate cancer: Regulation of NCAPH by antitumor miR‐199a/b‐3p.
- Authors
Arai, Takayuki; Kojima, Satoko; Yamada, Yasutaka; Sugawara, Sho; Kato, Mayuko; Yamazaki, Kazuto; Naya, Yukio; Ichikawa, Tomohiko; Seki, Naohiko
- Abstract
Objectives: To identify oncogenes regulated by micro‐ribonucleic acid, miR‐199a/b‐3p, in metastatic castration‐resistant prostate cancer. Methods: Advanced ribonucleic acid sequencing technologies were applied to construct a micro‐ribonucleic acid expression signature using metastatic castration‐resistant prostate cancer autopsy specimens. Ectopic expression of mature micro‐ribonucleic acids or small‐interfering ribonucleic acids were applied to functional assays for cancer cell lines. Genome‐wide gene expression and in silico database analyses were carried out to predict micro‐ribonucleic acid targets. Results: Ectopic expression of miR‐199a/b inhibited cancer cell aggressiveness. The gene coding for non‐structural maintenance of chromosomes condensin I complex subunit H was directly regulated by miR‐199a/b‐3p. High expression of condensin I complex subunit H was significantly associated with poor disease‐free survival by The Cancer Genome Atlas database analysis (P < 0.0001). Overexpression of condensin I complex subunit H was detected in hormone‐sensitive prostate cancer and castration‐resistant prostate cancer specimens, and knockdown assays showed that its expression enhanced cancer cell migration and invasive abilities. Conclusions: Small ribonucleic acid sequencing of metastatic castration‐resistant prostate cancer specimens showed the presence of several antitumor micro‐ribonucleic acids whose targets are involved in hormone‐sensitive prostate cancer and metastatic castration‐resistant prostate cancer pathogenesis. Condensin I complex subunit H seems to be a promising diagnostic marker and therapeutic target for this disease. Our approach, based on the roles of anti‐tumor micro‐ribonucleic acids and their targets, will contribute to an improved understanding of the molecular pathogenesis of hormone‐sensitive prostate cancer and metastatic castration‐resistant prostate cancer.
- Subjects
CASTRATION-resistant prostate cancer; RNA; CANCER cell migration; PROSTATE cancer; CARCINOGENESIS
- Publication
International Journal of Urology, 2019, Vol 26, Issue 4, p506
- ISSN
0919-8172
- Publication type
Article
- DOI
10.1111/iju.13911