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- Title
Immunological evaluation of invasive Lactobacillus plantarum co-expressing EtMIC2 and chicken interleukin-18 against Eimeria tenella.
- Authors
Zhang, Zan; Huang, Hai-Bin; Jiang, Yan-Long; Liu, Jing; Gao, Xing; Liu, Yang; Yang, Wen-Tao; Shi, Chun-Wei; Wang, Dan; Wang, Jian-Zhong; Kang, Yuan-Huan; Wang, Chun-Feng; Yang, Gui-Lian
- Abstract
Chicken coccidiosis is a protozoan parasitic disease that leads to considerable economic losses in the poultry industry. In this study, we used invasive Lactobacillus plantarum (L.P) expressing the FnBPA protein as a novel bacterial carrier for DNA delivery into epithelial cells to develop a live oral DNA vaccine. A fusion DNA vaccine co-expressing EtMIC2 and chicken IL-18 (chIL-18) was constructed and then delivered to the host by invasive L.P. Its efficacy against Eimeria tenella challenge was evaluated in chickens by examining the relative weight gain rate; caecal lesion score; OPG; anti-coccidial index (ACI); levels of EtMIC2 antibody, FnBPA, IL-4, IL-18, IFN-γ and SIgA; and proliferation ability and percentages of CD4+ and CD8+ splenocytes. The experimental results showed that chickens immunized with invasive L.P carrying the eukaryotic expression vector pValac-EtMIC2 (pValac-EtMIC2/pSIP409-FnBPA) had markedly improved immune protection against challenge compared with that of chickens immunized with non-invasive L.P (pValac-EtMIC2/pSIP409). However, invasive L.P co-expressing EtMIC2 with the chIL-18 vector exhibited the highest protection efficiency against E. tenella. These results indicate that invasive Lactobacillus-expressing FnBPA improved humoural and cellular immunity and enhanced resistance to E. tenella. The DNA vaccine delivered by invasive Lactobacillus provides a new concept and method for the prevention of E. tenella.
- Subjects
EIMERIA; EIMERIA tenella; LACTOBACILLUS plantarum; INTERLEUKIN-18; BACTERIAL DNA; DNA vaccines
- Publication
Parasitology Research, 2020, Vol 119, Issue 9, p2885
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-020-06745-w