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- Title
Development of an immunochromatographic test with recombinant BgSA1 for the diagnosis of Babesia gibsoni infection in dogs.
- Authors
Honglin Jia; Min Liao; Eunggoo Lee; Yoshifumi Nishikawa; Hisashi Inokuma; Hiromi Ikadai; Aya Matsuu; Ikuo Igarashi; Xuenan Xuan
- Abstract
Abstract An immunochromatographic test (ICT) using recombinant BgSA1 (rBgSA1) for the detection of antibodies againstBabesia gibsoniwas developed and evaluated. Only the serum samples collected from dogs infected withB. gibsoniwere positive in the ICT, but the serum samples from dogs infected with closely related parasites and from healthy dogs were negative. The specific antibodies could be detected in a dog experimentally infected withB. gibsoniat both the acute and chronic infection stages by the ICT. To evaluate the clinical application of the ICT, a total of 94 serum samples collected from domestic dogs in Japan were tested with the ICT and the previously established enzyme-linked immunosorbent assay (ELISA) with rBgSA1. Twenty-one of the tested samples (22.3%) were positive in both the ICT and the ELISA. The concordance between the ELISA and the ICT was found to be 95.8%. These results suggested that the ICT using rBgSA1 is rapid, simple, accurate, and suitable for the diagnosis ofB. gibsoniinfection of dogs in the field.
- Subjects
RECOMBINANT antibodies; BABESIA; SERUM; PARASITISM
- Publication
Parasitology Research, 2007, Vol 100, Issue 6, p1381
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-006-0392-8