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- Title
Localization of Estrogen Receptors in Rhesus Monkey Ovary.
- Authors
Hutz, R. J.; Wagner, N.; Krause, P.; Fisher, C.; Syed, N.; Dierschke, D. J.; Monnlaux, D.; Tomanek, M.
- Abstract
We have previously demonstrated that the exogenous administration of estradiol-17β (E2) to rhesus monkeys induces atresia of the dominant pre-ovulatory follicle (OF); and that this effect is mediated centrally, via the inhibition of follicle-stimulating hormone, and is also exerted directly at the level of the ovarian granulosa cell. We wished to investigate whether the local effect of E2 is transduced through interaction with the nuclear receptor for estrogen, particularly in light of certain evidence that suggests a general lack of estrogen receptor (E-R) in the rhesus monkey ovary, except in the germinal epithelium. In the present study, we evaluated the presence of E-R by both autoradiographic and immunocytochemical techniques. Frozen sections of ovaries from rhesus females were incubated in experiment 1 with either ³H-E2 or 125I-E2, in the presence or absence of excess, non-radioactive ligand or analogues diethylstilbestrol (DES) or the receptor antagonist 4-OH-tamoxifen (TAM). ³H-E2 binding was most in- tense over functional corpora lutea, and was reduced to background with excess DES; label was also evident over antral follicles. Image analysis showed specific binding of 125-E2 by ovaries. In experiment 2, cryostat sections were processed for immunocytochemical staining using the per-oxidase-anti-peroxidase (PAP) method and the H222 monoclonal antibody to the E-R. Intense, specific Label was observed over nuclei of germinal epithelium, but, additionally, for the first time, to granulosa cells of antral follicles and other compartments of the ovary.
- Subjects
ESTROGEN receptors; FOLLICLE-stimulating hormone; BLASTODERM; AUTORADIOGRAPHY; IMMUNOCYTOCHEMISTRY; RHESUS monkeys; OVARIES
- Publication
American Journal of Primatology, 1993, Vol 31, Issue 4, p299
- ISSN
0275-2565
- Publication type
Article
- DOI
10.1002/ajp.1350310406