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- Title
CTX-M-15 extended-spectrum -lactamase from Nigerian Klebsiella pneumoniae.
- Authors
Olusegun O. Soge; Anne Marie Queenan; Kayode K. Ojo; Bolanle A. Adeniyi; Marilyn C. Roberts
- Abstract
Objectives: In this study, extended-spectrum -lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria.Methods: The -lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the blaCTX-M genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNADNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants.Results: All 30 isolates produced at least one -lactamase. Seventeen of the isolates were resistant to cefotaxime, and had =100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have blaCTX-M genes that were associated with large plasmids (=58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 -lactamases. The ISEcp1 element was located upstream of blaCTX-M-15 in the same position as previously described. In addition, 23 of the isolates produced TEM -lactamases, 27 produced SHV -lactamases and four produced AmpC -lactamases.Conclusions: Thirty K. pneumoniae produced multiple -lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2006, Vol 57, Issue 1, p24
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dki429