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- Title
Immobilization of Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, on carbon supports.
- Authors
Maksimov, A.; Maksimova, Yu.; Kuznetsova, M.; Olontsev, V.; Demakov, V.
- Abstract
Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, was immobilized by adsorption on carbon supports differing in structure and porosity. The adsorption capacity of the supports towards cells, the substrate of the nitrile hydratase reaction (acrylonitrile), and the product (acrylamide) was studied. Also, the effect of immobilization on nitrile hydratase activity of bacteria was investigated, and the operational stability of the immobilized biocatalyst was determined. It was shown that crushed and granulated active coals were more appropriate for immobilization than fibrous carbon adsorbents.
- Subjects
NITRILES; GRAM-positive bacteria; POROSITY; ACRYLONITRILE; ACRYLAMIDE; ENZYMES; CARBON
- Publication
Applied Biochemistry & Microbiology, 2007, Vol 43, Issue 2, p173
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S000368380702007X