We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
THE SIMULTANEOUS DETERMINATION OF CANDESARTAN, AMLODIPINE AND HYDROCHLOROTHIAZIDE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, FROM A MIXTURE AND PHARMACEUTICAL FORMULATIONS.
- Authors
MOISEI, ANCA; TOTAN, MARIA; GLIGOR, FELICIA GABRIELA; CRĂCIUN, ISABELA; TODORAN, NICOLETA; CHIŞ, AURELIA ADRIANA; POPA, DANIELA ELENA
- Abstract
The aim of this paper consisted in the development and validation of a high performance liquid chromatography (HPLC) method for the determination of three antihypertensive substances from a mixture and pharmaceutical formulations. The three selected antihypertensive substances were candesartan, amlodipine and hydrochlorothiazide. The extraction and quantification of the constituents by HPLC was carried out using an analytical column, C18, and a mobile phase consisting in a mixture of buffer solution pH = 3.5 and methanol (15:85 v/v), at a flow rate of 1 mL/ min. The UV detection was performed at 240 nm for candesartan and amlodipine and at 270 nm for hydrochlorothiazide. The three compounds mentioned above were separated with good resolution, reproducibility and sensitivity under these conditions. The proposed HPLC method was applied to the analysis of the in-house dosage forms.
- Subjects
CANDESARTAN; AMLODIPINE; HYDROCHLOROTHIAZIDE; ANTIHYPERTENSIVE agents; HIGH performance liquid chromatography; THERAPEUTICS
- Publication
Farmacia, 2016, Vol 64, Issue 4, p612
- ISSN
0014-8237
- Publication type
Article