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- Title
Phytoconstituents from Polyscias guilfoylei leaves with histamine-release inhibition activity.
- Authors
Ashmawy, Naglaa S.; Gad, Haidy A.; Al-Musayeib, Nawal; El-Ahmady, Sherweit H.; Ashour, Mohamed L.; Singab, Abdel Nasser B.
- Abstract
Phytochemical investigation of Polyscias guilfoylei leaves extract (PGE) led to the isolation of nine compounds, that is, ent-labda-8(17),13-diene-15,18-diol (1), stigmasterol (2), spinasterol (3), N-(1,3-dihydroxyoctadecan-2-yl) palmitamide (4), panaxydiol (5), 3-O-β-d-glucopyranosylstigmasta-5,22-diene-3-β-ol (6), (8Z)-2-(2 hydroxypentacosanoylamino) octadeca-8-ene-1,3,4-triol (7), 4-hydroxybenzoic acid (8), and tamarixetin 3,7-di-O-α-L-rhamnopyranoside (9). Compound 4 is reported in this study for the first time in nature whereas compound 9 is reported for the second time. Structural elucidation of the compounds was carried out using Nuclear Magnetic Resonance and Electrospray Ionization coupled with Mass Spectrometry spectroscopic analyses. PGE and compounds 4 and 9 exhibited weak cytotoxicity against both MCF-7 and HCT-116 cell lines using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. The antimicrobial activity of PGE and compounds 4 and 9 was evaluated using the agar diffusion method. Escherichia coli was the most susceptible Gram-negative bacteria toward PGE with a minimum inhibitory concentration value of 9.76 μg/mL, whereas compounds 4 and 9 did not show any antimicrobial activity. Compound 4 exhibited promising inhibition of histamine release using U937 human monocytes with an IC50 value of 38.65 μg/mL.
- Subjects
ELECTROSPRAY ionization mass spectrometry; MASS analysis (Spectrometry); NUCLEAR magnetic resonance; LEAVES; GRAM-negative bacteria; BIOGENIC amines
- Publication
Zeitschrift für Naturforschung B: A Journal of Chemical Sciences, 2019, Vol 74, Issue 5, p145
- ISSN
0932-0776
- Publication type
Article
- DOI
10.1515/znc-2018-0167