We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts.
- Authors
Ko, Hyunjun; Bae, Jung-Hoon; Sung, Bong Hyun; Kim, Mi-Jin; Park, Soon-Ho; Sohn, Jung-Hoon
- Abstract
A functional sweetener, difructose anhydride IV (DFA IV), is enzymatically produced from sucrose via levan by levansucrase (LSRase) followed by levan fructotransferase (LFTase). Here, we have demonstrated a consolidated production system for the direct conversion of DFA IV from sucrose using the co-culture of two recombinant yeast strains secreting LSRase from Bacillus subtilis and LFTase from Arthrobacter ureafaciens, respectively. To ensure secretory production of the enzymes, target protein-specific translational fusion partners (TFP) were employed, and the selected strains produced 3.8 U/mL of LSRase and 16.0 U/mL LFTase activity into the fermentation broth. To optimise the direct production, sucrose concentration and cell ratios were investigated. In the optimised conditions, 64.3 g/L crude DFA IV was directly produced from 244.7 g/L sucrose using co-fermentation of recombinant yeasts. These results promise an efficient production titre, yield, and DFA IV productivity in an industrially applicable method.
- Subjects
SWEETENERS; ANHYDRIDES; LEVANSUCRASE; BACILLUS subtilis; ARTHROBACTER
- Publication
Scientific Reports, 2019, Vol 9, Issue 1, pN.PAG
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-019-52373-5