We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The CAP superfamily protein PsCAP1 secreted by Phytophthora triggers immune responses in Nicotiana benthamiana through a leucine‐rich repeat receptor‐like protein.
- Authors
Jiang, Haibin; Xia, Yeqiang; Zhang, Sicong; Zhang, Zhichao; Feng, Hui; Zhang, Qi; Chen, Xi; Xiao, Junhua; Yang, Sen; Zeng, Mengzhu; Chen, Zhaodan; Ouyang, Haibing; He, Xinyi; Sun, Guangzheng; Wu, Jinbin; Dong, Suomeng; Ye, Wenwu; Ma, Zhenchuan; Wang, Yan; Wang, Yuanchao
- Abstract
Summary: The role of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 (CAP) superfamily proteins in the innate immune responses of mammals is well characterized. However, the biological function of CAP superfamily proteins in plant–microbe interactions is poorly understood.We used proteomics and transcriptome analyses to dissect the apoplastic effectors secreted by the oomycete Phytophthora sojae during early infection of soybean leaves. By transiently expressing these effectors in Nicotiana benthamiana, we identified PsCAP1, a novel type of secreted CAP protein that triggers immune responses in multiple solanaceous plants including N. benthamiana. This secreted CAP protein is conserved among oomycetes, and multiple PsCAP1 homologs can be recognized by N. benthamiana.PsCAP1‐triggered immune responses depend on the N‐terminal immunogenic fragment (aa 27–151). Pretreatment of N. benthamiana with PsCAP1 or the immunogenic fragment increases plant resistance against Phytophthora. The recognition of PsCAP1 and different homologs requires the leucine‐rich repeat receptor‐like protein RCAP1, which associates with two central receptor‐like kinases BRI1‐associated receptor kinase 1 (BAK1) and suppressor of BIR1‐1 (SOBIR1) in planta.These findings suggest that the CAP‐type apoplastic effectors act as an important player in plant–microbe interactions that can be perceived by plant membrane‐localized receptor to activate plant resistance.
- Subjects
CAPPING proteins; NICOTIANA benthamiana; IMMUNE response; RECEPTOR-like kinases; PHYTOPHTHORA; PHYTOPHTHORA sojae; COAT proteins (Viruses)
- Publication
New Phytologist, 2023, Vol 240, Issue 2, p784
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.19194