We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
High Correlation between Results of the [1-[sup 13] C]-Phenylalanine Breath Test and Phenylalanine Hydroxylase (EC 1.14.16.1) Activity of the Liver in Rats.
- Authors
Ito, Asuka; Kohno, Tadashi; Hosoi, Isaburo; Ohshima, Junko; Ishii, Yukimoto; Asai, Satoshi; Ishikawa, Koichi; Shibata, Kunihiko
- Abstract
Background:[sup 13] CO[sub 2] is decreased in patients with end-stage liver disease by the [1-[sup 13] C]-phenylalanine breath test. Decreased [sup 13] CO[sub 2] is supposed to be caused by the decreased ability of the liver to oxidize phenylalanine. However, no direct evidence has been reported. Methods: The [1-[sup 13] C]-phenylalanine breath test was performed in galactosamine hepatitis rats (n = 14) and control rats (n = 8). Plasma phenylalanine concentration before intravenous administration of [1-[sup 13] C]-phenylalanine, the elimination rate of phenylalanine and the phenylalanine hydroxylase (PAH; EC 1.14.16.1) activity of the whole liver were examined. Results: Increase of [sup 13] CO[sub 2] in the breath [Δ[sup 13] CO[sub 2] (‰)] of galactosamine hepatitis rats 2 min after administration of [1-[sup 13] C]-phenylalanine was only 1/5 of that in control rats. The concentration of plasma phenylalanine and the elimination rate of plasma phenylalanine in hepatitis rats did not show significant differences compared to control rats. On the other hand, a clear difference in the activity of PAH was observed between hepatitis rats and control rats. Δ[sup 13] CO[sub 2] (‰) 2 min after administration of [1-[sup 13] C]-phenylalanine was highly correlated to the PAH activity of the whole liver (r = 0.917). Conclusion: It was strongly indicated that decreased Δ[sup 13] CO[sub 2 ] in hepatitis rats was the result of decreased activity of PAH.Copyright © 2001 S. Karger AG, Basel
- Subjects
BREATH tests; PHENYLALANINE; LIVER; HEPATITIS; RATS
- Publication
Digestion, 2001, Vol 63, Issue 2, p130
- ISSN
0012-2823
- Publication type
Article
- DOI
10.1159/000051881