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- Title
Role of Complement Activation and Mast Cell Degranulation in the Pathogenesis of Rapid Intestinal Ischemia/Reperfusion Injury in Rats.
- Authors
Andoh, Akira; Fujiyama, Yoshihide; Araki, Yoshio; Kimura, Toshio; Tsujikawa, Tomoyuki; Bamba, Tadao
- Abstract
The aim of this study is to define the putative role of complement activation and mucosal mast cell (MMC) degranulation in the pathogenesis of rapid ischemia-reperfusion (I/R) injury. We prepared complement activity-depleted rats by the administration of the anti-complementary agent K-76COONa. To assess the role of MMC degranulation, we used the MMC stabilizer MAR-99 and genetically mast cell-deficient Ws/Ws rats. Autoperfused segments of the jejunum were exposed to 60 min of ischemia, followed by 60 min reperfusion. The epithelial permeability was assessed by [sup 51] Cr-EDTA clearance rate, and the number of MMC was immunohistochemically assessed. I/R treatment induced a marked increase in mucosal permeability and MMC degranulation. The treatment with K-76COONa and MAR-99 significantly attenuated these changes. Furthermore, in Ws/Ws rats the increase in mucosal permeability and MMC degranulation was significantly attenuated. These findings indicate the role of complement activation and MMC activation in the pathogenesis of rapid intestinal I/R injury. A regulation of the complement activation and MMC degranulation may be one of the clinical strategies for prevention of I/R-induced mucosal injury.Copyright © 2001 S. Karger AG, Basel
- Subjects
MAST cells; INTESTINAL ischemia; INTESTINAL diseases; REPERFUSION injury; RATS
- Publication
Digestion, 2001, Vol 63, p103
- ISSN
0012-2823
- Publication type
Article
- DOI
10.1159/000051920