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- Title
Phytochemical profiles and in vitro anti-inflammatory properties of Perilla frutescens cv. Chookyoupjaso mutants induced by mutagenesis with γ-ray.
- Authors
Park, Yong; Lee, Young; Kang, Min; Lee, Hyo; Jin, Chang; Choi, Dae; Kim, Dong; Kang, Si-Yong; Kim, Wang-Geun; Jeong, Il
- Abstract
In earlier investigations, Perilla frutescens (L.) Britt. cv. Chookyoupjaso (CJC) mutants were obtained following mutagenesis induced by 200 Gy of γ-rays. The aim of this study was to compare the CJC and 6 P. frutescens (L.) Britt. cv. Chookyoupjaso mutant lines (CJMs), with respect to their phytochemical profiles and to evaluate anti-inflammatory properties by selecting the most bioactive CJM. The methanol extracts of CJMs were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Among them, CJM-45 showed significant inhibition of NO production. This extract was further partitioned using ethyl acetate (EtOAc), butanol (BuOH), and water. The EtOAc fraction (EF-cjm45) was evaluated for antiinflammatory activities. These results indicated that the EF-cjm45 reduced NO production by inhibiting inducible nitric oxide synthase (iNOS) expression. The EF-cjm45 treatment also significantly diminished expression of MCP-1 and IL-6. In the EF-cjm45, perillaketone, isoegomaketone, ursolic acid, kaempferol, and rosmarinic acid were also found. This study reveals the potential therapeutic use of bioactive CJM-45 and justifies the wide application for this plant in traditional medicine.
- Publication
Food Science & Biotechnology, 2010, Vol 19, Issue 2, p305
- ISSN
1226-7708
- Publication type
Article
- DOI
10.1007/s10068-010-0044-8