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- Title
Anther Culture in Rye: Improved Plant Regeneration Using Modified MS-Media.
- Authors
Daniel, G.
- Abstract
The use of androgenetic doubled haploids in rye breeding is still limited by low regeneration rates. In this study we tested the influence of two modified MS-media on the number of reacting anthers and on plant regeneration. Picloram and 2,4-D were used as auxin components in the induction media. The highest induction rates (reacting anthers per 100 cultivated anthers) and the highest regeneration rates (number of green plants per 100 cultivated anthers) were reached on a modified MS-medium containing Picloram as auxin source, regardless of the donor plant material. The comparison of the results obtained with the single cross 'SC 35' which contains genes of Secale vavilovii and the true Secale cereale donor forms clearly show the genotypic influence of donor plant material on induction and regeneration ability.
- Subjects
SECALE; ANTHER; HAPLOIDY; AUXIN; PLANT hormones; PLANT breeding
- Publication
Plant Breeding, 1993, Vol 110, Issue 3, p259
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.1993.tb00587.x