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- Title
Investigation of the regulatory effect of icariin on the hepatopancreas glycolipid metabolism and energy metabolism of female Eriocheir sinensis during exogenous vitellogenesis based on the transcriptomics and metabolomics.
- Authors
Zheng, Xiaochuan; Jiang, Weibo; Zhang, Ling; Espe, Marit; Abasubong, Kenneth P.; Zhang, Chunnuan; Zhang, Dingdong; Li, Xiangfei; Jiang, Guangzhen; Chi, Cheng; Liu, Wenbin
- Abstract
The Chinese mitten crab (Eriocheir sinensis) mainly depends on lipids and carbohydrates to meet the energy requirements for its growth and rapid ovarian development during the exogenous vitellogenesis. Therefore, this study aimed to explore the effects of dietary icariin supplement on the hepatopancreas' glycolipid metabolism and energy metabolism during this vital stage. Biochemical analysis revealed the apparent differential effects of icariin on the key indicators involving glycolipid metabolism. The functional mechanisms of icariin on glycolipid metabolism were systematically investigated using transcriptomic and metabolomic analysis. After 100 mg/kg icariin supplementation, fifty differential expression genes mainly involving in glycolysis, gluconeogenesis, fatty acid β‐oxidation, tricarboxylic acid cycle and mitochondrial oxidative phosphorylation pathway were found. In addition, forty differential metabolites mainly involving the metabolism process of pyruvate metabolism, fatty acid metabolism, arachidonic acid metabolism and steroid hormone synthesis were identified. Besides, the results of RT‐PCR and Western blot verified several key transcription factors related to glycolipid and energy metabolism; the results demonstrated that icariin could strengthen glycolipid metabolism and energy metabolism of E. sinensis by activating related genes. This would facilitate the further understanding of the metabolism and regulation mechanism of nutrition and energy during the exogenous vitellogenesis in crustaceans.
- Subjects
GLYCOLIPIDS; ENERGY metabolism; CHINESE mitten crab; GLYCOLYSIS; METABOLIC regulation; METABOLOMICS; VITELLOGENESIS
- Publication
Aquaculture Nutrition, 2021, Vol 27, Issue 6, p2066
- ISSN
1353-5773
- Publication type
Article
- DOI
10.1111/anu.13341