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- Title
miR-24 Regulates Apoptosis by Targeting the Open Reading Frame (ORF) Region of FAF1 in Cancer Cells.
- Authors
Wenming Qin; Yi Shi; Botao Zhao; Chengguo Yao; Li Jin; Jiexian Ma; Youxin Jin
- Abstract
Background: microRNAs (miRNAs) are small noncoding RNAs that regulate cognate mRNAs at the post-transcriptional stage. Several studies have shown that miRNAs modulate gene expression in mammalian cells by base pairing to complementary sites in the 3'-untranslated region (3'-UTR) of the target mRNAs. Methodology/Principal Findings: In the present study, miR-24 was found to target fas associated factor 1(FAF1) by binding to its amino acid coding sequence (CDS) region, thereby regulating apoptosis in DU-145 cells. This result supports an augmented model whereby animal miRNAs can exercise their effects through binding to the CDS region of the target mRNA. Transfection of miR-24 antisense oligonucleotide (miR-24-ASO) also induced apoptosis in HGC-27, MGC-803 and HeLa cells. Conclusions/Significance: We found that miR-24 regulates apoptosis by targeting FAF1 in cancer cells. These findings suggest that miR-24 could be an effective drug target for treatment of hormone-insensitive prostate cancer or other types of cancers. Future work may further develop miR-24 for therapeutic applications in cancer biology.
- Subjects
MESSENGER RNA; GENE expression; APOPTOSIS; CANCER treatment; AMINO acids; OLIGONUCLEOTIDES; DOSAGE forms of drugs; PROSTATE cancer; HORMONES
- Publication
PLoS ONE, 2010, Vol 5, Issue 2, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0009429