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- Title
Latency Associated Peptide Has In Vitro and In Vivo Immune Effects Independent of TGF-β1.
- Authors
Ali, Naeem A.; Gaughan, Alice A.; Orosz, Charles G.; Baran, Chris P.; McMaken, Sara; Wang, Yijie; Eubank, Timothy D.; Hunter, Melissa; Lichtenberger, Frank J.; Flavahan, Nicholas A.; Lawler, Jack; Marsh, Clay B.
- Abstract
Latency Associated Peptide (LAP) binds TGF-β1, forming a latent complex. Currently, LAP is presumed to function only as a sequestering agent for active TGF-β1. Previous work shows that LAP can induce epithelial cell migration, but effects on leukocytes have not been reported. Because of the multiplicity of immunologic processes in which TGF-β1 plays a role, we hypothesized that LAP could function independently to modulate immune responses. In separate experiments we found that LAP promoted chemotaxis of human monocytes and blocked inflammation in vivo in a murine model of the delayedtype hypersensitivity response (DTHR). These effects did not involve TGF-β1 activity. Further studies revealed that disruption of specific LAP-thrombospondin-1 (TSP-1) interactions prevented LAP-induced responses. The effect of LAP on DTH inhibition depended on IL-10. These data support a novel role for LAP in regulating monocyte trafficking and immune modulation.
- Subjects
PEPTIDE synthesis; IMMUNE response; IMMUNOTOXICOLOGY; CHEMOTAXIS; THROMBOSPONDINS; MONOCYTES
- Publication
PLoS ONE, 2008, Vol 3, Issue 4, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0001914