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- Title
Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts.
- Authors
Forte, Giancarlo; Pietronave, Stefano; Nardone, Giorgia; Zamperone, Andrea; Magnani, Eugenio; Pagliari, Stefania; Pagliari, Francesca; Giacinti, Cristina; Nicoletti, Carmine; Musaró, Antonio; Rinaldi, Mauro; Ribezzo, Marco; Comoglio, Chiara; Traversa, Enrico; Okano, Teruo; Minieri, Marilena; Prat, Maria; Di Nardo, Paolo
- Abstract
Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy. S TEM C ELLS 2011;29:2051-2061.
- Subjects
TISSUE engineering; CELLS; STEM cells; ANTIGENS; HEART cells; CELL suspensions
- Publication
Stem Cells, 2011, Vol 29, Issue 12, p2051
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1002/stem.763