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- Title
Generation of T Lineage Cells from Human Embryonic Stem Cells in a Feeder Free System.
- Authors
Galić, Zoran; Kitchen, Scott G.; Subramanian, Aparna; Bristol, Greg; Marsden, Matthew D.; Balamurugan, Arumugam; Kacena, Amelia; Yang, Otto; Zack, Jerome A.
- Abstract
Human embryonic stem cells (hESC) have the potential to revolutionize certain medical treatments, including T-cellbased therapies. However, optimal approaches to develop T cells from hESC are lacking. In this report, we show that T-cell progenitors can be derived from hESC cultured as embryoid bodies (EBs). These EB-derived T-cell progenitors give rise to phenotypically and functionally normal cells of the T lineage when transferred into human thymic tissue implanted in immunocompromised mice, suggesting that introduction of these progenitors into patients may also yield functional T cells. Moreover, hematopoietic progenitors demonstrating T-cell potential appeared to be CD45+/CD34+, resembling those found in normal bone marrow. In contrast to T cells developed from hESC cocultured on murine stromal cells, the EB-derived T cells also expressed normal levels of CD45. Importantly, the EB system eliminates the previous need for murine cocultures, a key impediment to developing a protocol for T-cell progenitor derivation suitable for clinical use. Furthermore, following lentiviral-mediated introduction of a vector expressing enhanced green fluorescent protein into hESC, stable transgene expression was maintained throughout differentiation, suggesting a potential for gene therapy approaches aimed at the augmentation of T-cell function or treatment of T-cell disorders.
- Subjects
EMBRYONIC stem cells; T cells; BONE marrow; GREEN fluorescent protein; TRANSGENE expression; GENE therapy; THERAPEUTICS
- Publication
Stem Cells, 2009, Vol 27, Issue 1, p100
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2008-0813