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- Title
Single-Cell Analysis of Ets-1 Transcription Factor Expression during Lymphocyte Activation and Apoptosis.
- Authors
Koskela, K.; Lassila, O.
- Abstract
Abstract The Ets-1 proto-oncogene is a prototype member of Ets family of transcription factors. It is preferentially expressed in lymphoid cells, where it is essential for the maintenance of the normal pool of resting T and B cells. We have investigated the protein expression of the Ets-1 transcription factor during the activation and apoptosis of T and B cells by flow cytometry and confocal microscopy. Cells of the thymus, spleen and bursa expressed high levels of Ets-1 protein, while resting peripheral blood mononuclear cells had lower Ets-1 expression. Activation and proliferation of T cells induced the upregulation of Ets-1. αβ-T cells were found to upregulate Ets-1 expression more than γδ-T cells. Increased Ets-1 protein expression was located predominantly in the perinuclear area. In contrast, during apoptosis, Ets-1 expression was downregulated. Collectively, our results indicate that Ets-1 expression can be accurately determined by flow cytometry and confocal microscopy. Ets-1 expression level and distribution are differentially controlled in resting, activated and apoptotic lymphocytes.
- Subjects
TRANSCRIPTION factors; APOPTOSIS; T cells; B cells; FLOW cytometry
- Publication
Scandinavian Journal of Immunology, 2003, Vol 57, Issue 1, p56
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2003.01192.x