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- Title
Human Microvascular Endothelial Cell Toxicity Caused by Brazilian Purpuric Fever-Associated Strains of Haemophilus influenzae Biogroup Aegyptius.
- Authors
Weyant, Robbin S.; Quinn, Frederick D.; Utt, Eric A.; Worley, Micah; George, Velma G.; Candal, Francisco J.; Ades, Edwin W.
- Abstract
An in vitro cytotoxicity model that uses an immortalized human microvascular endothelial cell line (HMEC-1) differentiates Brazilian purpuric fever (BPF)-associated Haemophilus infiuenzae biogroup aegyptius (HAE) strains from non-BPF-associated HAE strains. Toxic strains produced a characteristic HMEC-1 phenotype at an MOI of <1 bacterium/1000 tissue culture cells (TCC). Nontoxic strains required MOls of >1000 bacteria/TCC to produce an observable effect. The cytotoxic phenotype was characterized by the presence of large clumps of HMEC-1 cells, which detached from the monolayer within 48 h of inoculation by HAE cells. The cytotoxic phenotype was observed with 100% of BPF-associated HAE (40/40) and 14% of non-BPF-associated HAE (8/57; P < .001). The ability to study a BPF-associated phenotype in vitro using human microvascular cells should enhance our knowledge of BPF pathogenesis.
- Publication
Journal of Infectious Diseases, 1994, Vol 169, Issue 2, p430
- ISSN
0022-1899
- Publication type
Article