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- Title
Expression and Effects of IL-33 and ST2 in Allergic Bronchial Asthma: IL-33 Induces Eotaxin Production in Lung Fibroblasts.
- Authors
Kurokawa, Masatsugu; Matsukura, Satoshi; Kawaguchi, Mio; Ieki, Koushi; Suzuki, Shintaro; Odaka, Miho; Watanabe, Shin; Homma, Tetsuya; Sato, Masayuki; Yamaguchi, Munehiro; Takeuchi, Hiroko; Adachi, Mitsuru
- Abstract
Background: Interleukin (IL)-33, a new member of the IL-1 cytokine family, has been recognized as a key cytokine that enhances T helper 2-balanced immune regulation through its receptor ST2; however, the function and relationship of the IL-33 and ST2 pathways in bronchial asthma are still unclear. We investigated the cellular origin and regulation of IL-33 and ST2 in allergic bronchial asthma in vivo and in vitro. Methods: BALB/c mice were sensitized by intraperitoneal injections of ovalbumin (OVA) with alum. Mice were exposed to aerosolized 1% OVA for 30 min a day for 7 days. These mice were then challenged with aerosolized 1% OVA 2 days after the last day of exposure. After the OVA challenge, the mice were sacrificed and their lung tissues were obtained. Mouse lung fibroblasts were cultured and treated with IL-33 or IL-13. Results: The levels of IL-33 mRNA and IL-33 protein in lung tissue increased after the OVA challenge. Most IL-33-expressing cells were CD11c+ cells and epithelial cells, and many ST2-expressing cells were stained lung fibroblasts and inflammatory cells. IL-33 induced eotaxin/CCL11 production in lung fibroblasts. IL-33 and IL-13 synergistically induced eotaxin expression. Conclusions: IL-33 may contribute to the induction and maintenance of eosinophilic inflammation in the airways by acting on lung fibroblasts. IL-33 and ST2 may play important roles in allergic bronchial asthma. Copyright © 2011 S. Karger AG, Basel
- Subjects
ASTHMA; CYTOKINES; FIBROBLASTS; DENDRITIC cells; EOSINOPHIL disorders; INTRAPERITONEAL injections
- Publication
International Archives of Allergy & Immunology, 2011, Vol 155, p12
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000327259