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- Title
The effect of exogenous superoxide generator chemicals on sodA and flpA promoters expression in Lactococcus lactis.
- Authors
Akyol, Ismail
- Abstract
Oxidative stress is caused by superoxide anion or hydrogen peroxide under aerobic growth condition. Some bacteria could eliminate toxic species of oxygen using SodA enzyme. The effect of superoxide generating chemicals and hydrogen peroxide on sodA and flpA promoter activities were investigated in this study. The observation indicated that the flpA promoter activity increased with menadione addition in the flpB mutant but not flpA mutant backgrounds. Also sodA activity increased 4-, 7-, 50, and 27-fold in shaking (250 rpm), memadione, paraquat and plumbagin addition respectfully. Catalase enzyme supplementation had positive effect on bacterial growth during exponential phase of flpA mutants than flpB mutants and wild type. Exogenously, hydrogen peroxide addition was unable to increase either sodA promoter or SodA enzyme activity.
- Subjects
OXIDATIVE stress; SUPEROXIDES; BACTERIA; HYDROGEN peroxide; ANIONS; PARAQUAT; PLUMBAGINALES
- Publication
Journal of Cell & Molecular Biology, 2006, Vol 5, Issue 2, p87
- ISSN
1303-3646
- Publication type
Article