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- Title
Dissemination of the high-risk clone ST147 carbapenem-resistant Klebsiella pneumoniae from a local tertiary care hospital in South Korea.
- Authors
Jungsun Park; Eunkyung Shin; Min-Kyeong Kim; Seongjae Joo; Hyun Ju Jeong; Jaeil Yoo; Junyoung Kim
- Abstract
배경 The emergence of carbapenem-resistant Enterobacterales (CRE) infections are rapidly increased and represent a serious public threat. The Korea Disease Control and Prevention Agency (KDCA) has collect and test all CRE isolates to monitor antimicrobial resistance profiles and the associated resistance genes by Korean Antimicrobial Resistance Monitoring System. In 2020, a total of 16,883 carbapenemase pro- ducing Enterobacterales strains were collected, among these isolates, 21 strains were persistently isolated in a local tertiary care hospital. 방법 The collected isolates were tested antimicrobial susceptibility testing using the broth microdilution meth- od with customized Sensititre KRCDC2F, KORN panels. The genetic relationships were determined by pulsed-field gel electrophoresis (PFGE) after digestion of total chromosomal DNA with XbaI. Genomic characteristics were performed by both the short-read Illumina MiSeq and the long-read Oxford Nanopore MinION platforms. 결과 All isolates showed an identical antimicrobial resistant profile such as ampicillin, carbapenems, cephems, chloramphenicol, fluoroquinolone, macrolide and trimethoprim/sulfamethoxazole. Based on whole-genome analysis have confirmed all isolates shared a common genetic structure (ISAba125-IS630-blaNDM-1- bleMBL) and coharbored additional resistance determinants and no mutations in the QRDRs. All K. pneumoniae isolates belonged to ST147 and K64 capsular type, which were carried on IncFIB replicon plasmids. Compared with 21 isolates in one hospital, the strain divided into five PFGE patterns; these isolates were found to be highly related by SNP and cgMLST to isolates (≤27 SNPs and ≤5 alleles differences). 결론 These finding suggested that NDM-1-producing K. pneumoniae ST147 may have been introduced to common resource that implying the result of nosocomial transmission. Thus, the strengthen monitoring is necessary to prevent the dissemination for high-risk clone and adequate infection control measures against spread of transmissible of national and transnational especially underlying mechanisms should be needed.
- Subjects
SOUTH Korea; KLEBSIELLA pneumoniae; CARBAPENEM-resistant bacteria; TERTIARY care; PULSED-field gel electrophoresis; MICROBIAL sensitivity tests; DRUG resistance in microorganisms; BETA lactamases
- Publication
Infection & Chemotherapy, 2022, Vol 54, pS254
- ISSN
2093-2340
- Publication type
Article