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- Title
A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma.
- Authors
Warttinger, U.; Giese, C.; Harenberg, J.; Holmer, Erik; Krämer, Roland
- Abstract
Heparins are widely used anticoagulant drugs. The current monitoring practice for heparin in plasma, such as the chromogenic anti-factor Xa assay, relies on heparin-triggered activation of antithrombin, an inhibitor of coagulation proteases. Such assays are not applicable to the detection of non-anticoagulant heparins, an emerging class of drug candidates for therapeutic applications unrelated to anticlotting activity. This study describes the application of a commercially available fluorescent probe assay (Heparin Red) for the direct and sensitive detection of the 'chemical' heparin in plasma, independent of any anticoagulant activity. The quantification range is about 0-5 μg/mL for both unfractionated heparin (corresponding to 0-1 IU/mL) and the low molecular weight heparin enoxaparin. The Heparin Red assay is of particular value for the quantification of non-anticoagulant heparins, as exemplified by the low molecular weight heparin derivative tafoxiparin and a N-desulfated-N-reacetylated heparin. Heparin octa- and decasaccharides are also detected.
- Subjects
HEPARIN; IMMUNOASSAY; ANTICOAGULANTS; BLOOD coagulation; PROTEOLYTIC enzymes
- Publication
Analytical & Bioanalytical Chemistry, 2016, Vol 408, Issue 28, p8241
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-016-9940-y