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- Title
Introgression of a novel Thinopyrum intermedium St-chromosome-specific HMW-GS gene into wheat.
- Authors
Li, Guang-Rong; Liu, Cheng; Li, Cheng-Hui; Zhao, Jin-Mei; Zhou, Li; Dai, Gang; Yang, En-Nian; Yang, Zu-Jun
- Abstract
Thinopyrum intermedium has been hybridized extensively with wheat ( Triticum aestivum L.) and several genes for disease resistance have been introgressed to cultivated wheat. However, there are very few reports about the Th. intermedium-derived seed storage protein genes which have been transferred into a wheat background by chromosome manipulation. Our aim is to identify several wheat- Th. intermedium ssp. trichophorum derivatives, and document these lines by genomic in situ hybridization (GISH), molecular markers and seed storage protein analysis. We found that a novel Th. intermedium 1St#2 chromosome-specific high-molecular-weight glutenin subunit (HMW-GS) was transferred to the wheat- Thinopyrum derivative lines. The genomic sequence of the Thinopyrum-derived HMW-GS was characterized and designated Glu- 1St#2x, since it resembled x-type glutenins in both the N-terminal domain and C-terminal domain. It is much shorter than that of reported HMW-GS genes. The Glu- 1St#2x sequence was successfully expressed in Escherichia coli and resulted in the identical weight to the native protein. The GISH and newly developed chromosome Thinopyrum-specific DNA markers enabled physically location of Glu- 1St#2x to the region FL0.60-1.00 on Th. intermedium 1St#2L chromosome arm. Phylogenetic analysis revealed that the Glu- 1St#2x evolved earlier than other x-type HMW-GS homoeologues in modern wheat genomes. The effect of Glu- 1St#2x on protein content, sodium dodecyl sulphate sedimentation value and improvement of solvent retention capacity in wheat background suggested that Th. intermedium chromosome 1St#2 may have potential for improvement of wheat end-product quality.
- Subjects
MOLECULAR weights; GLUTELINS; IN situ hybridization; WHEAT quality; GENES
- Publication
Molecular Breeding, 2013, Vol 31, Issue 4, p843
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-013-9838-8