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- Title
The CpG island of the FMR-1 gene is methylated differently among embryonic tissues: implication for prenatal diagnosis.
- Authors
Iida, Taku; Nakahori, Yutaka; Tsutsumi, Osamu; Taketani, Yuji; Nakagome, Yasuo; Iida, T; Nakahori, Y; Tsutsumi, O; Taketani, Y; Nakagome, Y
- Abstract
We studied the methylation status of the CpG island of the FMR-1 (fragile X syndrome) gene to recognize the possibility of its prenatal diagnosis with early pregnant subjects. Southern hybridization using EcoRI/BssHII restriction enzymes double digestion was performed in the brain and chorionic villi of 8th week embryos, and the placenta and cord blood of newborns. No methylation of the FMR-1 gene occurred in both of the tissues examined in males, while 50% of the cells in females were methylated in both the brain and the cord blood, indicating that methylation occurs with inactivation of the X-chromosome in accordance with the literature. However, there was no methylation in either the chorionic villi or placenta in female as well as in males. Some extra-embryonic tissues such as the chorionic villi and the placenta escape X-chromosome FMR-1 gene inactivation and it can be the exception in the lyonization. To assess the methylation status in prenatal diagnosis, precautions are needed and they are not suitable for prenatal diagnosis.
- Subjects
METABOLISM in the fetus; DIAGNOSIS of fragile X syndrome; BRAIN metabolism; DNA analysis; DNA metabolism; NUCLEOTIDE metabolism; BRAIN; CHORIONIC villi; COMPARATIVE studies; CORD blood; FRAGILE X syndrome; HUMAN reproduction; IMMUNOBLOTTING; RESEARCH methodology; MEDICAL cooperation; METHYLATION; NUCLEOTIDE separation; PLACENTA; PRENATAL diagnosis; RESEARCH; EVALUATION research
- Publication
Human Reproduction, 1994, Vol 9, Issue 8, p1471
- ISSN
0268-1161
- Publication type
journal article