We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
貂源肺炎克雷伯菌的分离鉴定及胞外分泌 蛋白的核酸酶活性分析.
- Authors
贾艳艳; 李 琦; 王晓利; 牛俊辉; 毛 静; 廖成水; 张昊恺; 张明亮
- Abstract
In order to identify the pathogens causing mink pneumonia, a suspected strain of Klebsiella pneumoniae was isolated from clinical death cases. The nuclease activity of extracellular protein of Klebsiella pneumoniae was detected by agarose gel electrophoresis, and the effects of temperature, pH value and metal ions on the activity of nuclease were investigated. The results showed that Klebsiella pneumoniae was identified by microscopic examination and PCR. The exocrine protein of Klebsiella pneumoniae showed the activity of nuclease for degradation of λDNA. The optimal temperature range was 30—37°C, and the optimum pH value was 7.0. The activity of nuclease was weak under high temperature and alkaline conditions. Univalent metal ions (Na+ and K+) and some divalent metal ions (Ca2+, Ba 2+, Ni 2+, Zn2+ and Cu2+ ) had no significant effect on the nuclease activity of extracellular secretory protein of Klebsiella pneumoniae. The 5. 00—10. 00 mmol / L Co 2+ and Fe3+ and 10. 00 mmol / L Mg2+ and Mn 2+ could promote the activity of extracellular nuclease cleavaging λDNA. In this study, a Klebsiella pneumoniae strain was isolated from clinical mink pneumonia,and its exocrine protein had nuclease activity.
- Subjects
KLEBSIELLA pneumoniae; METAL ions; GEL electrophoresis; TEMPERATURE effect; HIGH temperatures; CALCIUM ions; ALKALI metal ions; CALCIUM
- Publication
Journal of Henan Agricultural Sciences, 2021, Vol 50, Issue 3, p142
- ISSN
1004-3268
- Publication type
Article
- DOI
10.15933/j.cnki.1004-3268.2021.03.019