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- Title
Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO.
- Authors
Bommer, Martin; Coates, Leighton; Dau, Holger; Zouni, Athina; Dobbek, Holger
- Abstract
The PsbO protein of photosystem II stabilizes the active-site manganese cluster and is thought to act as a proton antenna. To enable neutron diffraction studies, crystals of the β-barrel core of PsbO were grown in capillaries. The crystals were optimized by screening additives in a counter-diffusion setup in which the protein and reservoir solutions were separated by a 1% agarose plug. Crystals were cross-linked with glutaraldehyde. Initial neutron diffraction data were collected from a 0.25 mm3 crystal at room temperature using the MaNDi single-crystal diffractometer at the Spallation Neutron Source, Oak Ridge National Laboratory.
- Subjects
PHOTOSYSTEMS; NEUTRON diffraction; GLUTARALDEHYDE
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2017, Vol 73, Issue 9, p525
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X17012171