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- Title
Establishment of a fluorescence resonance energy transfer-based bioassay for detecting dioxin-like compounds.
- Authors
Chi-Iou Lin; Chun-Hung Hsieh; Shiao-Ying Lee, Stanley; Wei-Shan Lee; Gou-Ping Chang-Chien; Chien-Yuan Pan; Hsinyu Lee
- Abstract
Dioxins comprise a group of compounds which contain a double aromatic ring-like structure. They are among the most prevalent and toxic environmental pollutants. Accumulation of dioxins in human tissues poses a potential threat to human health. Currently, analytical chemical procedures dominate dioxin-detection protocols. In this study, we established a fluorescence resonance energy transfer (FRET)-based dioxin-detection bioassay. Aryl hydrocarbon receptor (AHR) and AHR nuclear translocator (ARNT) fused-cyan fluorescent protein (CFP) and -yellow fluorescent protein (YFP) constructed were transiently co-transfected into rat hepatoma cell line, H4IIEC3 cells. Our results showed that no FRET signals were detected in AHR-CFP- and ARNT-YFP-transfected H4IIEC3 cells. However, dioxin treatments upregulated FRET signals in these transfected cells in a dose-dependent manner. This work highlighted the potential of FRET technique in the detection of dioxin-like compounds.
- Subjects
DIOXINS; POLLUTANTS; CELL lines; CELLS; BIOLOGICAL assay
- Publication
Journal of Biomedical Science, 2008, Vol 15, Issue 6, p833
- ISSN
1021-7770
- Publication type
Article
- DOI
10.1007/s11373-008-9267-6