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- Title
Molecular Cloning and Functional Expression of the Human Golgi UDP-N-Acetylglucosamine Transporter1.
- Authors
Ishida, Nobuhiro; Yoshioka, Shigemi; Chiba, Yasunori; Takeuchi, Makoto; Kawakita, Masao
- Abstract
We have cloned the human UDP-N-acetylglucosamine (UDP-GlcNAc) transporter cDNA, which was recognized through a homology search in the expressed sequence tags database (dbEST) based on its similarity to the human UDP-galactose transporter. The chromosomal location of the UDP-GlcNAc transporter gene was assigned to chromosome lp21 by fluorescence in situ hybridization (FISH). The transporter was expresed ubiquitously in every tissue so far examined. Expression of the transporter cDNA in CHO-K1 cells in its native and in a C-terminally HA-tagged form indicated that the human UDP-GlcNAc transporter was localized in the Golgi apparatus. The membrane vesicles prepared from yeast cells expressing the cDNA product exhibited UDP-GlcNAc-specific transporting activity. Comparison among UDP-galactose, CMP-sialic acid, and UDP-GlcNAc transporters from several organisma enabled us to identify residues highly conserved among the transporters and residues specific for each group of transporters.
- Subjects
MOLECULAR cloning; GENE expression; CARBOXYLIC acids; SIALIC acids; GALACTOSE
- Publication
Journal of Biochemistry, 1999, Vol 126, Issue 1, p68
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a022437