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- Title
Co-Expression of Yeast Amber Suppressor tRNATyr and Tyrosyl-tRNA Synthetase in Escherichia coli: Possibility to Expand the Genetic Code1.
- Authors
Ohno, Satoshi; Yokogawa, Takashi; Fujii, Isao; Asahara, Haruichi; Inokuchi, Hachiro; Nishikawa, Kazuya
- Abstract
An efficient system was developed for the co-expression of a yeast tRNATyr/tyrosyl-tRNA synthetase (TyrRS) pair in Escherichia coli. Analysis of suppression patterns using several sets of E. coli and λ phage mutants indicated that the expressed yeast suppressor tRNATyr was aminoacylated only with tyrosine by its cognate yeast TyrRS and not by E. coli TyrRS or other aminoacyl-tRNA synthetases. This extra tRNA/TyrRS pair is expected to be a key bridgehead for developing an in vivo system for the site-directed incorporation of unnatural amino acids into proteins.
- Subjects
YEAST research; AMINO acids; LIGASES; FOODBORNE diseases; ESCHERICHIA coli
- Publication
Journal of Biochemistry, 1998, Vol 124, Issue 6, p1065
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a022221