We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
DNA methylation regulates the expression of the negative transcriptional regulators ID2 and ID4 during OPC differentiation.
- Authors
Tiane, Assia; Schepers, Melissa; Riemens, Renzo; Rombaut, Ben; Vandormael, Patrick; Somers, Veerle; Prickaerts, Jos; Hellings, Niels; van den Hove, Daniel; Vanmierlo, Tim
- Abstract
The differentiation of oligodendrocyte precursor cells (OPCs) into myelinating oligodendrocytes is the prerequisite for remyelination in demyelinated disorders such as multiple sclerosis (MS). Epigenetic mechanisms, such as DNA methylation, have been suggested to control the intricate network of transcription factors involved in OPC differentiation. Yet, the exact mechanism remains undisclosed. Here, we are the first to identify the DNA-binding protein inhibitors, Id2 and Id4, as targets of DNA methylation during OPC differentiation. Using state-of-the-art epigenetic editing via CRISPR/dCas9-DNMT3a, we confirm that targeted methylation of Id2/Id4 drives OPC differentiation. Moreover, we show that in the pathological context of MS, methylation and gene expression levels of both ID2 and ID4 are altered compared to control human brain samples. We conclude that DNA methylation is crucial to suppress ID2 and ID4 during OPC differentiation, a process that appears to be dysregulated during MS. Our data do not only reveal new insights into oligodendrocyte biology, but could also lead to a better understanding of CNS myelin disorders.
- Subjects
DNA methylation; OLIGODENDROGLIA; DNA-binding proteins; MYELIN oligodendrocyte glycoprotein; TRANSCRIPTION factors; GENE expression; METHYLATION
- Publication
Cellular & Molecular Life Sciences, 2021, Vol 78, Issue 19/20, p6631
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-021-03927-2