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- Title
Heat shock protein 70-2 (HSP70-2) is a novel therapeutic target for colorectal cancer and is associated with tumor growth.
- Authors
Jagadish, Nirmala; Parashar, Deepak; Gupta, Namita; Agarwal, Sumit; Suri, Vaishali; Kumar, Rajive; Suri, Vitusha; Sadasukhi, Trilok Chand; Gupta, Anju; Ansari, Abdul S.; Lohiya, Nirmal Kumar; Suri, Anil
- Abstract
<bold>Background: </bold>Colorectal cancer (CRC) is the third leading cause of cancer related deaths worldwide both in men and women. Our recent studies have indicated an association of heat shock protein 70-2 (HSP70-2) with bladder urothelial carcinoma. In the present study, we investigated the association of HSP70-2 with various malignant properties of colorectal cancer cells and clinic-pathological features of CRC in clinical specimens.<bold>Methods: </bold>HSP70-2 mRNA and protein was investigated expression by RT-PCR, immunohistochemistry, immunofluorescence, flow cytometry and Western blotting in CRC clinical specimens and COLO205 and HCT116 cell lines. Plasmid-based gene silencing approach was employed to study the association of HSP70-2 with various malignant properties of COLO205 and HCT116 cells in in vitro and with tumor progression in in vivo COLO205 human xenograft mice model.<bold>Results: </bold>HSP70-2 expression was detected in 78 % of CRC patients irrespective of various stages and grades by RT-PCR and IHC. Our analysis further revealed that HSP70-2 expression was detected in both COLO205 and HCT116 cell lines. Ablation of HSP70-2 expression resulted in reduced cellular growth, colony forming ability, migratory and invasive ability of CRC cells. In addition, ablation of HSP70-2 expression showed significant reduction in tumor growth in COLO205 human xenograft in in vivo mouse model.<bold>Conclusion: </bold>Collectively, our results indicate that HSP70-2 is associated with CRC clinical specimens. In addition, down regulation of HSP70-2 expression reduces cellular proliferation and tumor growth indicating that HSP70-2 may be a potential therapeutic target for CRC treatment.
- Subjects
HSP70 heat-shock proteins; COLON cancer patients; COLON cancer treatment; TUMOR growth; MESSENGER RNA; IMMUNOHISTOCHEMISTRY; IMMUNOFLUORESCENCE; PROTEIN metabolism; ANIMALS; ANTHROPOMETRY; CELL lines; CELL physiology; CELLS; CELL motility; COLON tumors; GENES; MICE; POLYMERASE chain reaction; PROTEINS; RECTUM tumors; RESEARCH funding; WESTERN immunoblotting; REVERSE transcriptase polymerase chain reaction; TUMOR treatment
- Publication
BMC Cancer, 2016, Vol 16, p1
- ISSN
1471-2407
- Publication type
journal article
- DOI
10.1186/s12885-016-2592-7