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- Title
Valproate Reduced Synaptic Activity Increase Induced by 4-Aminopyridine at the Hippocampal CA3-CA1 Synapse.
- Authors
Martín, Eduardo D.; Pozo, Miguel A.
- Abstract
Purpose: We investigated the effects of valproate (VPA) on excitatory synaptic transmission changes induced by 4-aminopyridine (4-AP) to determine whether the antiepileptic effects shown by VPA can be ascribed to a modulation of spontaneous excitatory postsynaptic currents (sEPSCs) in the CA3-CA1 synapse. Methods: Rat hippocampal slices were prepared and maintained in vitro with standard methods. Whole-cell current and voltage-clamp recordings were obtained from CA1 pyramidal neurons by using the "blind" patch-clamp technique in an immersion recording chamber. Increase in the spontaneous excitatory synaptic activity was induced by addition of 4-AP to the medium. Results: Perfusion with VPA significantly counteracted the increase of frequency and amplitude of the sEPSCs induced by application of 4-AP and suppressed the epileptiform activity. Conclusions: We conclude that VPA decreases the 4-AP-induced enhancement of excitatory synaptic activity at the CA3CA1 synapse, and that this reduction of excitation input to CA1 contributes to the anticonvulsant effects of VPA.
- Subjects
VALPROIC acid; AMINOPYRIDINES; EPILEPSY; HIPPOCAMPUS (Brain); NEUROPHYSIOLOGY; BRAIN diseases
- Publication
Epilepsia (Series 4), 2004, Vol 45, Issue 5, p436
- ISSN
0013-9580
- Publication type
Article
- DOI
10.1111/j.0013-9580.2004.58303.x