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- Title
Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication.
- Authors
Yan, Bingpeng; Chu, Hin; Yang, Dong; Sze, Kong-Hung; Lai, Pok-Man; Yuan, Shuofeng; Shuai, Huiping; Wang, Yixin; Kao, Richard Yi-Tsun; Chan, Jasper Fuk-Woo; Yuen, Kwok-Yung
- Abstract
Lipids play numerous indispensable cellular functions and are involved in multiple steps in the replication cycle of viruses. Infections by human-pathogenic coronaviruses result in diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifestations. Understanding how cellular lipids may modulate the pathogenicity of human-pathogenic coronaviruses remains poor. To this end, we utilized the human coronavirus 229E (HCoV-229E) as a model coronavirus to comprehensively characterize the host cell lipid response upon coronavirus infection with an ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS)-based lipidomics approach. Our results revealed that glycerophospholipids and fatty acids (FAs) were significantly elevated in the HCoV-229E-infected cells and the linoleic acid (LA) to arachidonic acid (AA) metabolism axis was markedly perturbed upon HCoV-229E infection. Interestingly, exogenous supplement of LA or AA in HCoV-229E-infected cells significantly suppressed HCoV-229E virus replication. Importantly, the inhibitory effect of LA and AA on virus replication was also conserved for the highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV). Taken together, our study demonstrated that host lipid metabolic remodeling was significantly associated with human-pathogenic coronavirus propagation. Our data further suggested that lipid metabolism regulation would be a common and druggable target for coronavirus infections.
- Subjects
CORONAVIRUS diseases; CORONAVIRUSES; LIPID metabolism; MICROBIAL virulence; HIGH performance liquid chromatography; ARACHIDONIC acid
- Publication
Viruses (1999-4915), 2019, Vol 11, Issue 1, p73
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v11010073