We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Bode Phase Angle Signaling of a TB Disease Biomarker.
- Authors
Sidwaba, Unathi; Januarie, Kaylin Cleo; Mini, Sixolile; Mokwebo, Kefilwe Vanessa; Iwuoha, Emmanuel; Feleni, Usisipho
- Abstract
Tuberculosis (TB) is a worldwide burden whose total control and eradication remains a challenge due to factors including false positive/negative diagnoses associated with the poor sensitivity of the current diagnostics in immune-compromised and post-vaccinated individuals. As these factors complicate both diagnosis and treatment, the early diagnosis of TB is of pivotal importance towards reaching the universal vision of a TB-free world. Here, an aptasensor for signaling an interferon gamma (IFN-γ) TB biomarker at low levels is reported. The aptasensor was assembled through gold–thiol interactions between poly(3,4-propylenedioxythiophene), gold nanoparticles, and a thiol-modified DNA aptamer specific to IFN-γ. The aptasensor sensitively detected IFN-γ in spiked pleural fluid samples with a detection limit of 0.09 pg/mL within a linear range from 0.2 pg/mL to 1.2 pg/mL. The good performance of the reported aptasensor indicates that it holds the potential for application in the early diagnosis of, in addition to TB, various diseases associated with IFN-γ release in clinical samples.
- Subjects
TUBERCULOSIS; INTERFERON gamma; BIOMARKERS; GOLD nanoparticles; DETECTION limit; APTAMERS; INTERFERONS
- Publication
Molecules, 2023, Vol 28, Issue 24, p8100
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28248100