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- Title
Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine.
- Authors
Zorina, Maria; Dotsenko, Victor V.; Nesterenko, Pavel N.; Temerdashev, Azamat; Dmitrieva, Ekaterina; Feng, Yu-Qi; Atapattu, Sanka N.
- Abstract
Dopamine, adrenaline and octopamine are small polar molecules that play a vital role in regulatory systems. In this paper, phthalylglycyl chloride was proposed as a derivatization agent for octopamine, adrenaline and dopamine determination in urine for the first time. The derivatization procedure facilitated the use of reversed-phase liquid chromatography with positive electrospray ionization–high-resolution mass spectrometry. An LC-HRMS method was developed that provided quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes. The 95–97% yield of derivates was observed after a 10 min derivatization with phthalylglycyl chloride at pH 6.5 and 30 °C. The proposed method was successfully applied to the analysis of human urine samples. The obtained results were compared with those of conventional derivatization procedures with 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride.
- Subjects
OCTOPAMINE; LIQUID chromatography-mass spectrometry; DERIVATIZATION; DOPAMINE; SMALL molecules; CHLORIDES; ADRENALINE; ELECTROSPRAY ionization mass spectrometry
- Publication
Molecules, 2023, Vol 28, Issue 7, p2900
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28072900