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- Title
Flow cytometric analysis of genome size variation in somePassifloraspecies.
- Authors
Magalhães Souza, Margarete; Palomino, Guadalupe; Nair Santana Pereira, Telma; Gonzaga Pereira, Messias; Pio Viana, Alexandre
- Abstract
Nuclear genome size variation was studied in eight taxa ofPassiflora.Nuclear DNA content was estimated by flow cytometry of nuclei stained by propidium iodide. 2C DNA content ranged from 3.16–5.36 pg for diploids and 1.83 pg for tetraploid. Differences in nuclear genome size were observed amongPassifloraspecies (pg):P. suberosa1.83,P. edulisf.edulis3.16,P. edulisf.flavicarpa(Brazil) 3.19,P. edulisf.flavicarpa(Mexico) 3.21,P. mucronata3.40,Passiflora edmundoi3.43,P. laurifolia3.88,P. giberti3.92,P. quadrangularis5.36, the largest value being up to 192% greater than the smallest. The means of 2C DNA content were compared by the Tukey test, and the differences in genome size permitted the recognition of five taxa groups. The result was the same for the means 2C genome size (Mbp) values. The genetic parameters were studied with their respective estimators, phenotypic variance (), genotypic variability (), and the genotypic determination index (H2). The genotypic determination index presented high magnitude estimates (greater than 99%) emphasizing the reliability of the results and demonstrating the efficiency of determining the DNA content in the species using only one leaf per plant.Passifloraspecies show great phenotypic variability and have different geographic distribution that might implicate in genetic diversity.
- Subjects
CYTOMETRY; GENOMES; PASSIFLORA; DNA; GENETICS
- Publication
Hereditas, 2004, Vol 141, Issue 1, p31
- ISSN
0018-0661
- Publication type
Article
- DOI
10.1111/j.1601-5223.2004.01739.x