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- Title
Development of oral morphine rapid test by using yolk immunoglobulin.
- Authors
Rajabibazl, Masoumeh; Rasaee, Mohammad Javad; Tiraihi, Taki; Reza, Ali; Sohi, Naderi; Hallaj, Shima
- Abstract
Avian antibody technology has been used in diagnosis and therapy A'lab-on-a-chip' system for detecting morphine in an oral fluid sample was described. An antibody against morphine was obtained in an avian system. The inununogen (M-G6-HS-BSA) was injected into Leghorn chicken. Furthermore, this antibody could be used in developing an enzyme immunoassay and immunochromatography to detect morphine in a specific and sensitive manner. The reported rapid test was an optical immunoassay test for detection of morphine. Out of 50 specimens obtained from addicted persons showed positive results in a urine test following standard morphine detection protocols. All samples showed salivary morphine presence using the newly developed assay. A simple, rapid and high yield procedure (40 mg/egg) was developed for IgY anti- morphine. Oral morphine rapid tests in all samples were able to detect morphine 0 to 8 hours after the routine drug use. The sensitivity of present assay was found to be around 65 ng/mL, with a high correlation with the standard kit for the detection of morphine in urine. Oral morphine immunochromatography was a rapid test with a high positive predictive value. The kit provided a reliable method for detection of morphine where laboratory support and personnel were limited.
- Publication
Journal of Applied Research, 2010, Vol 10, Issue 3, p133
- ISSN
1537-064X
- Publication type
Journal Article