We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Relevance of the two-component sensor protein CiaH to acid and oxidative stress responses in Streptococcus pyogenes.
- Authors
Ichiro Tatsuno; Masanori Isaka; Ryo Okada; Yan Zhang; Tadao Hasegawa
- Abstract
Background The production of virulence proteins depends on environmental factors, and two-component regulatory systems are involved in sensing these factors. We previously established knockout strains in all suspected two-component regulatory sensor proteins of the emm1 clinical strain of S. pyogenes and examined their relevance to acid stimuli in a natural atmosphere. In the present study, their relevance to acid stimuli was re-examined in an atmosphere containing 5% CO2. Results The spy1236 (which is identical to ciaH py ) sensor knockout strain showed significant growth reduction compared with the parental strain in broth at pH 6.0, suggesting that the Spy1236 (CiaHpy) two-component sensor protein is involved in acid response of S. pyogenes. CiaH is also conserved in Streptococcus pneumoniae, and it has been reported that deletion of the gene for its cognate response regulator (ciaR pn ) made the pneumococcal strains more sensitive to oxidative stress. In this report, we show that the spy1236 knockout mutant of S. pyogenes is more sensitive to oxidative stress than the parental strain. Conclusions These results suggest that the two-component sensor protein CiaH is involved in stress responses in S. pyogenes.
- Publication
BMC Research Notes, 2014, Vol 7, Issue 1, p1
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/1756-0500-7-189