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- Title
Fertility preservation in cancer patients.
- Authors
Abtahi N. S.; Ebrahimi B.; Rezazadeh Valojerdi M.; Fathi R.
- Abstract
Introduction: Fertility preservation before gonadotoxic treatments is necessary in cancer patients. Ovarian tissue cryopreservation is the only available option for prepubertal girls and women who cannot delay cancer treatment or when ovarian stimulation is contraindicated. Materials and Methods: Human ovarian tissue biopsies were obtained from six women (29-40 years old) who were candidates for oophorectomy for benign gynecologic conditions. Their medulla were removed, cortical parts were thinned and then cut into 10x5x1 mm strips. Cortical strips were vitrified in two steps by using vitrification solutions including; VI [HTCM as base medium (BM)+ 7.5% DMSO+ 7.5% EG+ 20% HAS] and V2 (BM+ 15% DMSO+ 15% EG+ 0.25M sucrose+ 20% HSA) for 15 and 10 min respectively. Morphology of ovarian tissue and enclosed follicles were compared in control and vitrification groups by histological assessment. Results: Ovarian stromal integrity, granulosa cells distribution, follicular population and morphology were well preserved in vitrified-warmed strips as fresh one. Oocyte degeneration, vacuolization and granulation were rarely observed in vitrified-warmed ovarian tissues. Primordial follicles were more distributed in cortical region in both groups. The mean percentage of intact follicles (primordial, primary and pre antral) are higher in control groups compared to vitrification one but the difference was not significant. Conclusion: Accordingly, used vitrification method could be suitable for human ovarian tissue preservation in cancer patients.
- Subjects
HUMAN fertility; CANCER patients
- Publication
Iranian Journal of Reproductive Medicine, 2015, p31
- ISSN
1680-6433
- Publication type
Abstract