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- Title
In vitro conservation of Bacopa monnieri (L.) using mineral oil.
- Authors
Sharma, Neelam; Satsangi, Richa; Pandey, Ruchira; Singh, Rakesh; Kaushik, Nutan; Tyagi, Rishi
- Abstract
Bacopa monnieri (L.) Wettst., a traditional Indian medicinal plant with high commercial potential, is used as a potent nervine tonic. A slow growth protocol was developed for medium-term conservation using mineral oil (MO) overlay. Nodal segments of B. monnieri (two genotypes; IC249250, IC468878) were conserved using MO for 24 months. Single node explants were implanted on MS medium supplemented with 0.2 mg l BA and were covered with MO. Subculture duration could be significantly enhanced from 6 to 24 months, on the above medium. Normal plants regenerated from conserved cultures were successfully established in soil. On the basis of 20 random amplified polymorphic DNA and 5 inter-simple sequence repeat primers analyses and bacoside A content using HPLC, no significant reproducible variation was observed between the controls and in vitro-conserved plants. The results demonstrate the feasibility of using MO for medium-term conservation of B. monnieri germplasm without any adverse genetical and biochemical effects.
- Subjects
INDIA; PLANT conservation; MINERAL oils; MEDICINAL plants; TONICS (Medicinal preparations); PLANT DNA
- Publication
Plant Cell, Tissue & Organ Culture, 2012, Vol 111, Issue 3, p291
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-012-0194-x