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- Title
Possible pathogenic role of T helper type 9 cells and interleukin ( IL)-9 in atopic dermatitis.
- Authors
Ma, L.; Xue, H.‐B.; Guan, X.‐H.; Shu, C.‐M.; Zhang, J.‐H.; Yu, J.
- Abstract
T helper type 9 (Th9) cells are a novel identified subset of CD4+ T helper cells, which could partly contribute to allergic inflammation, while the precise contribution of Th9 cells in atopic dermatitis ( AD) remains unknown. We aimed to explore the possible role of Th9 cells in AD pathogenesis. The Th9 cell percentage, transcription factor PU.1 and cytokine interleukin ( IL)-9 mRNA levels, as well as IL-9 serum concentration in peripheral circulation, were measured in AD patients, psoriasis patients and healthy controls. The Th9 cell percentage, PU.1 and IL-9 expression levels of AD patients were all increased significantly compared with the other two control groups ( P < 0·01), and correlated positively with SCORing Atopic Dermatitis index, serum immunoglobulin ( Ig)E and thymus- and activation-regulated chemokine ( TARC) levels ( P < 0·05). In simple AD patients and AD patients complicated by allergic rhinitis or asthma, there were no significant differences in the Th9 cell percentage, PU.1 and IL-9 expression levels between them. At the same time, IL-9 and vascular endothelial growth factor ( VEGF) mRNA levels were detected in AD lesions and normal skin samples, which were both distinctly elevated in AD lesions, and there was a positive association between them ( P < 0·01). Keratinocytes were cultured with IL-9 stimulation and the secretion of VEGF was detected. IL-9 can promote the secretion of VEGF by keratinocytes in a time- and dose-dependent manner. In conclusion, the expansion of the Th9 cell subset, up-regulation of the PU.1 transcription factor and increased secretion of the IL-9 cytokine may contribute to the pathogenesis of AD, which may be supported by the increased release of VEGF by keratinocyes after IL-9 stimulation.
- Subjects
T helper cells; INTERLEUKINS; CD4 antigen; INFLAMMATION; ATOPIC dermatitis; IMMUNOGLOBULIN E; VASCULAR endothelial growth factors; COMPARATIVE studies; PATIENTS
- Publication
Clinical & Experimental Immunology, 2014, Vol 175, Issue 1, p25
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/cei.12198