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- Title
Characteristics of the cell proliferation profile of activated rat hepatic stellate cells in vitro in contrast to their fibrogenesis activity.
- Authors
Suzuki, Chieko; Kayano, Kozo; Uchida, Kouichi; Sakaida, Isao; Okita, Kiwamu; Suzuki, C; Kayano, K; Uchida, K; Sakaida, I; Okita, K
- Abstract
<bold>Purpose: </bold>Alterations in the kinetics of hepatic stellate cells (HSCs) after the cells are activated once have not been well documented. We investigated the characteristic profiles of cell proliferation of once-activated HSCs in contrast to the in fibrogenesis activity.<bold>Methods: </bold>HSCs from male Wistar rats were submitted to primary culture for 14 days and to secondary culture for 7 days. The potential for cell proliferation was evaluated by the number of the cells in G2/M phase, based on flow cytometric analysis of the cell cycle. The fibrogenesis activity was assessed by Northern blot analysis of the expression of type I and type III procollagen mRNA.<bold>Results: </bold>The number of HSCs in G2/M phase was maintained at a low level in primary culture after 6 days, while a significantly (P < 0.05) elevated number of HSCs in G2/M phase was observed on days 3 to 4. In secondary culture, the number of HSCs in G2/M phase was also consecutively maintained at a decreased level. By contrast, HSCs showed progressively increased type I and type III procollagen mRNA expression during the experimental periods of primary culture.<bold>Conclusions: </bold>These results clearly demonstrated consecutively decreased proliferative activity, evaluated by the potential for cell mitogenesis, in once-activated HSCs, in contrast to their progressively increased fibrogenesis activity.
- Subjects
LIVER cells; CELL cycle; MESSENGER RNA
- Publication
Journal of Gastroenterology, 2001, Vol 36, Issue 5, p322
- ISSN
0944-1174
- Publication type
journal article
- DOI
10.1007/s005350170098