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- Title
Bone marrow mammaglobin expression as a marker of graft-versus-tumor effect after reduced-intensity allografting for advanced breast cancer.
- Authors
Bregni, M.; Fleischhauer, K.; Bernardi, M.; Pescarollo, A.; Guggiari, E.; Lunghi, F.; Deola, S.; Scaramuzza, S.; Re, F.; Setola, E.; Monari, M.; Mazzi, B.; Servida, P.; Corradini, P.; Peccatori, J.
- Abstract
We assessed mammaglobin (MMG) gene expression in bone marrow (BM) aspirates from patients with advanced breast cancer who had received a reduced-intensity conditioning and stem cell allografting, in order to detect a graft-versus-tumor effect on micrometastatic disease. Nine patients received a reduced-intensity conditioning with fludarabine, cyclophosphamide, and thiotepa, followed by peripheral blood allografting from HLA-identical sibling donors. Nested RT-PCR analysis with sequence-specific primers for MMG was carried out on a monthly basis on BM samples. Three patients had MMG-positive BM, four patients had MMG-negative BM before allografting, and two were undetermined. In two patients, a clinical response after allografting (partial remission) occurred concurrently with the clearance of MMG expression, at a median of 6 months after allografting, following immune manipulation. In two patients, a prolonged stable disease and negative MMG expression occurred after day +360 from allografting. In two patients, progression of the disease was associated with MMG RT-PCR changing from negative to positive. In one case, a disease response occurring after donor lymphocyte infusion and grade II acute GVHD was heralded by negativization of MMG expression. Although preliminary, these data suggest that a graft-versus-breast cancer effect is detectable on micrometastatic BM disease.Bone Marrow Transplantation (2006) 37, 311–315. doi:10.1038/sj.bmt.1705248; published online 9 January 2006
- Subjects
BREAST cancer; GENE expression; STEM cell transplantation; BONE marrow; HOMOGRAFTS; GRAFT versus host disease
- Publication
Bone Marrow Transplantation, 2006, Vol 37, Issue 3, p311
- ISSN
0268-3369
- Publication type
Article
- DOI
10.1038/sj.bmt.1705248